Cytokines are proteins secreted by cells of innate and acquired immunity, produced in response to various antigens and responsible for mediating several function of these cells. Our study evaluated the profile of cytokines interleukin 5 (IL-5) and interferon gamma (IFN-γ), induced in lymphocytes of broiler chickens in response to secondary fungal metabolites ochratoxin A (OTA) and deoxynivalenol (DON) at concentrations of 0.001, 0.01, 0.1 and 1 μg/ml. The quantification of the cytokines was analysed at 24, 48 and 72 h after incubation with mycotoxins, using real-time PCR (qRT-PCR). The results obtained showed that OTA induced mRNA synthesis of IL-5 at concentrations 0.001, 0.1 and 1 μg/ml after 24 h of lymphocyte incubation, while at 48 h only the expression of the IL-5 cytokine at a concentration of 1 μg/ml (P<0.05) was detected. DON in a concentration of 1 μg/ml induced the expression of IL-5 in the lymphocytes only at 48 h post-incubation period (P<0.05). Regarding IFN-γ, gene expression was not observed in the lymphocytes of broiler chickens incubated with OTA and DON. The data obtained represent a profile of response mediated by T helper 2 cells to the exposure of broiler chicken immune cells to different concentrations of OTA and DON.
RESEARCH ARTICLE
In vitro effect of ochratoxin A and deoxynivalenol on the expression of interleukin 5 and interferon-gamma in broiler chicken lymphocytes
C. Lautert Related information
1Setor de Micologia, Universidade Federal do Rio Grande do Sul, 91540-000, Porto Alegre, RS, Brazil
, L. Ferreiro Related information1Setor de Micologia, Universidade Federal do Rio Grande do Sul, 91540-000, Porto Alegre, RS, Brazil
, M.I. Azevedo Related information1Setor de Micologia, Universidade Federal do Rio Grande do Sul, 91540-000, Porto Alegre, RS, Brazil
, S.A. Botton Related information2Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil
, J.T. Santos Related information3Departamento de Clínica de Grandes Animais, Hospital Veterinário, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil
, P.B.D. Gonçalves Related information3Departamento de Clínica de Grandes Animais, Hospital Veterinário, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil
, K.B. Schlemmer Related information4Departamento de Microbiologia e Parasitologia, Laboratório de Pesquisas Micológicas (LAPEMI), Universidade Federal de Santa Maria, Avenida Roraima 1000, Cidade Universitária, Bairro Camobi, 97105-900, Santa Maria, RS, Brazil
, V.S. Machado Related information2Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil
, J.M. Santurio Related information4Departamento de Microbiologia e Parasitologia, Laboratório de Pesquisas Micológicas (LAPEMI), Universidade Federal de Santa Maria, Avenida Roraima 1000, Cidade Universitária, Bairro Camobi, 97105-900, Santa Maria, RS, Brazil
*janio. santurio@gmail. com
*janio.
World Mycotoxin Journal: 9
(2)- Pages: 299 - 304
Published Online: August 31, 2015
Abstract
Keywords: immunotoxicology, cytotoxicity, fungal metabolites, poultry
2023 Journal Impact Factor
2.0
source: Journal Impact Factor 2023™ from Clarivate™
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