Enterohaemorrhagic Escherichia coli O157:H7 and adherent-invasive Escherichia coli are two groups of enteric bacterial pathogens associated with haemorrhagic colitis and Crohn's Disease, respectively. Bacterial contact with host epithelial cells stimulates an immediate innate immune response designed to combat infection. In this study, immune responses of human epithelial cells to pathogens, either alone or in combination with probiotic bacteria were studied. Industrially prepared Lactobacillus helveticus strain R0052 was first examined by microarray analysis and then compared to broth-grown strains of R0052 and Lactobacillus rhamnosus strain GG using quantitative realt-time polymerase chain reaction. Results showed host immune activation responses increased following pathogen exposure, which were differentially ameliorated using probiotics depending on both the preparation of probiotics employed and conditions of exposure. These findings provide additional support for the concept that specific probiotic strains serve as a promising option for use in preventing the risk of enteric bacterial infections.
Research-Article
Immune signalling responses in intestinal epithelial cells exposed to pathogenic Escherichia coli and lactic acid-producing probiotics
N.K. Ho Related information
1 Department of Laboratory Medicine and Pathobiology, University of Toronto, 27 King's College Circle, Toronto, ON M5S 1A1, Canada
2 Department of Pediatrics, Hospital for Sick Children, 555 University Avenue, Toronto, ON M5G 1X8, Canada
, S.P. Hawley Related information2 Department of Pediatrics, Hospital for Sick Children, 555 University Avenue, Toronto, ON M5G 1X8, Canada
2 Department of Pediatrics, Hospital for Sick Children, 555 University Avenue, Toronto, ON M5G 1X8, Canada
, J.C. Ossa Related information2 Department of Pediatrics, Hospital for Sick Children, 555 University Avenue, Toronto, ON M5G 1X8, Canada
, O. Mathieu Related information3 Lallemand Human Nutrition, 6100 Avenue Royalmount, Montreal, QC H4P 2R2, Canada
, T.A. Tompkins Related information3 Lallemand Human Nutrition, 6100 Avenue Royalmount, Montreal, QC H4P 2R2, Canada
, K.C. Johnson-Henry Related information2 Department of Pediatrics, Hospital for Sick Children, 555 University Avenue, Toronto, ON M5G 1X8, Canada
, P.M. Sherman Related information1 Department of Laboratory Medicine and Pathobiology, University of Toronto, 27 King's College Circle, Toronto, ON M5S 1A1, Canada
2 Department of Pediatrics, Hospital for Sick Children, 555 University Avenue, Toronto, ON M5G 1X8, Canada
2 Department of Pediatrics, Hospital for Sick Children, 555 University Avenue, Toronto, ON M5G 1X8, Canada
Beneficial Microbes: 4
(2)- Pages: 195 - 209
Published Online: February 26, 2013
Abstract
Keywords: pathogen, epithelia, probiotics, microarray, immune, gene expression
2022 Journal Impact Factor
5.4
source: Journal Impact Factor 2023™ from Clarivate™
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