This review summarises developments in the determination of mycotoxins over a period between mid-2014 and mid-2015. In tradition with previous articles of this series, analytical methods to determine aflatoxins, Alternaria toxins, ergot alkaloids, fumonisins, ochratoxins, patulin, trichothecenes and zearalenone are covered in individual sections. Advances in proper sampling strategies are discussed in a dedicated section, as are new methods used to analyse botanicals and spices and newly developed LC-MS based multi-mycotoxin methods. The critical review aims to briefly discuss the most important developments and trends in mycotoxin determination as well as to address shortcomings of current methodologies.