An analytical method for quantifying ochratoxin A (OTA) in pet foods using high-performance liquid chromatography was developed, and an inter-laboratory study was conducted. OTA was extracted from samples with aqueous acetonitrile. The extract was purified by an immunoaffinity column, OCHRAKING, and analysed by high performance liquid chromatography with fluorescence detection. The limits of quantification by this method were 2 µg/kg for dry and semidry pet food and 1 µg/kg for wet type pet food. The calibration curve showed linearity in the range of 0.5-50 ng/ml (equivalent to 1-100 µg/kg for wet type pet food). The mean recoveries of OTA spiked at 1-5 µg/kg were in the range of 83.0-106% and relative standard deviations of the in-house method validation were 2.6-6.8%. The mean recoveries, repeatability, reproducibility and the Horwitz ratios for OTA from the inter-laboratory validation study were 75.6-83.1%, 3.5-6.1%, 5.0-15.0% and 0.23-0.68, respectively.
RESEARCH ARTICLE
Development and inter-laboratory study of a method for quantifying ochratoxin A in pet foods
T. Wada Related information
1Chemicals Evaluation and Research Institute Japan (CERI), CERI Tokyo, Environmental Technology Department, 1600, Shimotakano, Sugito-machi, Kitakatsushika-gun, Saitama 345-0043, Japan.
3Graduate School of Science and Engineering, Saitama University 255, Shimo-Okubo, Sakura, Saitama 338-8570, Japan.
*wada-takeharu@ceri. jp
, H. Saito Related information3Graduate School of Science and Engineering, Saitama University 255, Shimo-Okubo, Sakura, Saitama 338-8570, Japan.
*wada-takeharu@ceri.
2Food and Agricultural Materials Inspection Center (FAMIC) Fertilizer and Feed Inspection Department, Saitama Shintoshin National Government Building, Kensato Building 2-1, Shintoshin, Chuo-ku, Saitama-shi, Saitama 330-9731, Japan.
, K. Aoyama Related information2Food and Agricultural Materials Inspection Center (FAMIC) Fertilizer and Feed Inspection Department, Saitama Shintoshin National Government Building, Kensato Building 2-1, Shintoshin, Chuo-ku, Saitama-shi, Saitama 330-9731, Japan.
, S. Saito Related information3Graduate School of Science and Engineering, Saitama University 255, Shimo-Okubo, Sakura, Saitama 338-8570, Japan.
, M. Shibukawa Related information3Graduate School of Science and Engineering, Saitama University 255, Shimo-Okubo, Sakura, Saitama 338-8570, Japan.
World Mycotoxin Journal: 10
(1)- Pages: 53 - 61
Published Online: June 13, 2016
Abstract
Keywords: mycotoxin, HPLC, fluorescence detector, immunoaffinity column
2023 Journal Impact Factor
2.0
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