Soybean and soy meal samples collected during the harvest season 2008-2009 in the soybean-growing area of Córdoba Province in Argentina were analysed for T-2 and HT-2 toxins occurrence. These mycotoxins were detected using HPLC analysis with fluorescence detection after derivatisation with 1-anthronylnitrile and immunoaffinity column clean-up. Characteristics of in-house validated method such as accuracy, precision, detection and quantification limits were defined by means of recovery test with spiked soybean and soy meal samples. Mean recoveries for T-2 within the spiking range 125-500 µg/kg, were 90.9 and 81.3% for soybean and soy meal, respectively with a withinlaboratory relative standard deviation <10%. Analysis of samples spiked with HT-2 in the same range gave a mean recovery of 70.2 and 77.5% for soybean and soy meal, respectively, with relative standard deviations <12%. The limit of detection for the method was 25 µg/kg for T-2 and HT-2, based on a signal-to-noise ratio 3:1 and the limit of quantification was established as three times the detection limit. Out of 64 samples, only two soybean samples showed contamination with A-type trichothecenes evaluated. Confirmatory analyses of the contaminated samples were performed by LC-MS/MS. This study demonstrated low incidences and levels of T-2 and HT-2 in soybean harvested among the areas in the Cordoba Province.
Survey of T-2 and HT-2 toxins in soybean and soy meal from Argentina using immunoaffinity clean-up and high performance liquid chromatography
G. Barros Related information
1 Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36 Km 601, 5800 Río Cuarto, Córdoba, Argentina
2 Member of the Research Career of CONICET, Argentina
, D. García Related information2 Member of the Research Career of CONICET, Argentina
1 Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36 Km 601, 5800 Río Cuarto, Córdoba, Argentina
, M. Oviedo Related information1 Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36 Km 601, 5800 Río Cuarto, Córdoba, Argentina
, M. Ramirez Related information1 Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36 Km 601, 5800 Río Cuarto, Córdoba, Argentina
2 Member of the Research Career of CONICET, Argentina
, A. Torres Related information2 Member of the Research Career of CONICET, Argentina
1 Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36 Km 601, 5800 Río Cuarto, Córdoba, Argentina
2 Member of the Research Career of CONICET, Argentina
, V. Lattanzio Related information2 Member of the Research Career of CONICET, Argentina
3 National Research Council, Institute of Sciences of Food Production CNR-ISPA, Via G. Amendola 122/O, 70126 Bari, Italy
, M. Pascale Related information3 National Research Council, Institute of Sciences of Food Production CNR-ISPA, Via G. Amendola 122/O, 70126 Bari, Italy
, S. Chulze Related information1 Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36 Km 601, 5800 Río Cuarto, Córdoba, Argentina
2 Member of the Research Career of CONICET, Argentina
2 Member of the Research Career of CONICET, Argentina
World Mycotoxin Journal: 4
(2)- Pages: 189 - 197
Published Online: April 18, 2011
Abstract
Keywords: T-2 toxin, HT-2 toxin, soybean, HPLC-FLD, immunoaffinity column, 1-anthronylnitrile
2023 Journal Impact Factor
2.0
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