Here we present a summary of the generation of dog Single-molecule Real-time Isoform Sequencing (Iso-Seq) data, and its application for transcriptome annotation. Iso-Seq libraries from six tissues were sequenced, and read clean-up was implemented with IsoSeq V3 tools. A high retention rate in demultiplexing and refining steps, as well as a high mapping rate (90%) of Full-length non-concatemer (FLNC) reads, guaranteed enough useful information for subsequent analyses. Finally, 17,766-45,170 clustered transcripts from the six libraries were aligned to the ROS_Cfam_1.0, a male Labrador retriever dog reference genome assembly. In terms of annotation, we identified 14,059 to 26,263 transcripts and 4,985 to 10,457 genes with the sequence data in each of the libraries, with 32 to 68% unique transcripts being expressed. Coding transcripts in each tissue are expressed in a relatively stable manner, from 60-70%. This study provides an improved dog genome annotation resource and offers new insights into tissue-specific transcriptome pattern.
Proceedings of 12th World Congress on Genetics Applied to Livestock Production (WCGALP)
Technical and species orientated innovations in animal breeding, and contribution of genetics to solving societal challenges
EditorsR.F. Veerkamp and Y. de Haas
Published: 2022 Pages: 3364
eISBN: 978-90-8686-940-4
Book Type: Conference Proceedings
752. Applications of single-molecule real-time isoform sequencing (Iso-Seq) for unravelling complexity of dog transcriptomes
W. Zhang Related information
1The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, United Kingdom.
*Corresponding author 1: wengang. zhang@ed. ac. uk
, L. Eory Related information*Corresponding author 1: wengang.
1The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, United Kingdom.
, M. Salavati Related information1The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, United Kingdom.
, E. Clark Related information1The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, United Kingdom.
, J. Smith Related information1The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, United Kingdom.
, A.L. Archibald Related information1The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, United Kingdom.
, J.J. Schoenebeck Related information1The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, United Kingdom.
*Corresponding author 2: jeff. schoenebeck@roslin. ed. ac. uk
*Corresponding author 2: jeff.
Pages: 3097 - 3100
Published Online: February 09, 2023
Abstract: