The aim of the research was to compare the survival of a blend of five probiotic strains (2 bifidobacteria and 3 lactobacilli) in a capsule within capsule (Duocap®) containing Ahiflower® oil, as compared to the strains in the powder (with or without Ahiflower oil), or the strains when present in the inner capsule only. This was tested in a validated, dynamic in vitro model of the stomach and small intestine (TIM-1), simulating human adults. Experiments were performed both in the gastric compartment of the model, as well as in the complete system (stomach + small intestine). Survival of the strains after transit through the gastric compartment in the Duocap capsule was higher by about a factor of 1.5 compared to the other 3 variables. In these gastric experiments, the Ahiflower oil did not seem to have an additional benefit, in the sense that it did not increase survival over the strains alone. After transit through the complete gastrointestinal tract survival was approximately 2-fold higher for the strains within the Duocap capsule, compared to the strains within the inner capsule or the powder. In these experiments, Ahiflower oil did have an additional benefit. The survival of the strains in the combination of powder with Ahiflower oil showed a similar survival as that of the Duocap, although in the first few hours of the experiments survival of both species lagged behind, and only caught up at the end of the test. In conclusion, the developed capsule-in-capsule technology increased the amount of viable cells in the upper gastrointestinal tract, mainly due to the presence of the polyunsaturated fatty acids contained in the outer capsule, which particularly protected the blend of probiotics in the small intestine.
RESEARCH ARTICLE
Survival of a probiotic-containing product using capsule-within-capsule technology in an in vitro model of the stomach and small intestine (TIM-1)
K. Venema Related information
1Centre for Healthy Eating & Food Innovation (HEFI), School of Nutrition and Translational Research in Metabolism (NUTRIM), Department of Human Biology, Maastricht University – campus Venlo, St. Jansweg 20, 5928 RC Venlo, the Netherlands.
*Corresponding author: k. venema@maastrichtuniversity. nl
, J. Verhoeven Related information*Corresponding author: k.
1Centre for Healthy Eating & Food Innovation (HEFI), School of Nutrition and Translational Research in Metabolism (NUTRIM), Department of Human Biology, Maastricht University – campus Venlo, St. Jansweg 20, 5928 RC Venlo, the Netherlands.
, C. Beckman Related information2Nouri Life, 801 Blacklawn Rd STE N, Conyers, GA 30012, USA.
, D. Keller Related information3Keller Consulting Group, 2417 Beachwood Blvd., Beachwood, OH 44122, USA.
Beneficial Microbes: 11
(4)- Pages: 403 - 409
Published Online: April 08, 2020
Abstract
Keywords: probiotic, survival, capsule, Lactobacillus, Bifidobacterium
2022 Journal Impact Factor
5.4
source: Journal Impact Factor 2023™ from Clarivate™
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